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Strategies for Bispecific Single Chain Antibody in Cancer Immunotherapy.

ABSTRACT: Genetic engineering has resulted in more than 50 recombinant bispecific antibody formats over the past two decades. Bispecific scFv antibodies represent a successful and promising immunotherapy platform that retargets cytotoxic T cells to tumor cells, with one scFv directed to tumor-associated antigens and the other to T cells. Based on this antibody construct, strategies for both specific tumor targeting and T cell activation are reviewed here. Three distinct types of tumor antigens are considered to optimize specificity and safety in bispecific scFv based treatment: cancer-testis antigens, neo-antigens and virus-associated antigens. In terms of T cell activation, although CD3 has been widely applied in bispecific scFvs being developed, CD28 and CD137 among co-stimulatory signals are also ideal candidates to be evaluated. Besides, LIGHT and HIV-Tat101 have drawn much attention as their potential roles in modulating antitumor responses.

SUBMITTER: Zhou SJ

PROVIDER: S-EPMC5688922 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Strategies for Bispecific Single Chain Antibody in Cancer Immunotherapy.

Zhou Shu-Juan SJ Wei Jia J Su Shu S Chen Fang-Jun FJ Qiu Yu-Dong YD Liu Bao-Rui BR

Journal of Cancer 20171017 18

Genetic engineering has resulted in more than 50 recombinant bispecific antibody formats over the past two decades. Bispecific scFv antibodies represent a successful and promising immunotherapy platform that retargets cytotoxic T cells to tumor cells, with one scFv directed to tumor-associated antigens and the other to T cells. Based on this antibody construct, strategies for both specific tumor targeting and T cell activation are reviewed here. Three distinct types of tumor antigens are conside ...[more]

PMID: 29151956

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