Ontology highlight
ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC56922 | biostudies-literature | 2001 Aug
REPOSITORIES: biostudies-literature
Jiang Y Y Cypess A M AM Muse E D ED Wu C R CR Unson C G CG Merrifield R B RB Sakmar T P TP
Proceedings of the National Academy of Sciences of the United States of America 20010821 18
We prepared a stable cell line expressing the glucagon receptor to characterize the effect of G(s)-coupled receptor stimulation on extracellular signal-regulated protein kinase 1/2 (ERK1/2) activity. Glucagon treatment of the cell line caused a dose-dependent increase in cAMP concentration, activation of cAMP-dependent protein kinase (PKA), and transient release of intracellular calcium. Glucagon treatment also caused rapid dose-dependent phosphorylation and activation of mitogen-activated prote ...[more]