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Rat embryonic stem cells produce fertile offspring through tetraploid complementation.


ABSTRACT: Pluripotency of embryonic stem cells (ESCs) can be functionally assessed according to the developmental potency. Tetraploid complementation, through which an entire organism is produced from the pluripotent donor cells, is taken as the most stringent test for pluripotency. It remains unclear whether ESCs of other species besides mice can pass this test. Here we show that the rat ESCs derived under 2i (two small molecule inhibitors) conditions at very early passages are able to produce fertile offspring by tetraploid complementation. However, they lose this capacity rapidly during culture due to a nearly complete loss of genomic imprinting. Our findings support that the naïve ground state pluripotency can be captured in rat ESCs but also point to the species-specific differences in its regulation and maintenance, which have implications for the derivation and application of naïve pluripotent stem cells in other species including human.

SUBMITTER: Li TD 

PROVIDER: S-EPMC5692557 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Rat embryonic stem cells produce fertile offspring through tetraploid complementation.

Li Tian-Da TD   Feng Gui-Hai GH   Li Yu-Fei YF   Wang Mei M   Mao Jun-Jie JJ   Wang Jia-Qiang JQ   Li Xin X   Wang Xue-Peng XP   Qu Bin B   Wang Le-Yun LY   Zhang Xin-Xin XX   Wan Hai-Feng HF   Cui Tong-Tong TT   Wan Cong C   Liu Lei L   Zhao Xiao-Yang XY   Hu Bao-Yang BY   Li Wei W   Zhou Qi Q  

Proceedings of the National Academy of Sciences of the United States of America 20171023 45


Pluripotency of embryonic stem cells (ESCs) can be functionally assessed according to the developmental potency. Tetraploid complementation, through which an entire organism is produced from the pluripotent donor cells, is taken as the most stringent test for pluripotency. It remains unclear whether ESCs of other species besides mice can pass this test. Here we show that the rat ESCs derived under 2i (two small molecule inhibitors) conditions at very early passages are able to produce fertile of  ...[more]

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