CGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.
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ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC5693259 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Publications
cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.
Wang Han H Liu Jonathan J Gharib Shahla S Chai Cynthia M CM Schwarz Erich M EM Pokala Navin N Sternberg Paul W PW
Nature methods 20161219 2
The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic ...[more]
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