Ontology highlight
ABSTRACT:
SUBMITTER: Zhao F
PROVIDER: S-EPMC5693742 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Zhao Feifei F Matt Sarah M SM Bu Jiexun J Rehrauer Owen G OG Ben-Amotz Dor D McLuckey Scott A SA
Journal of the American Society for Mass Spectrometry 20170711 10
Electro-osmotically induced Joule heating in theta tips and its effect on protein denaturation were investigated. Myoglobin, equine cytochrome c, bovine cytochrome c, and carbonic anhydrase II solutions were subjected to electro-osmosis in a theta tip and all of the proteins were denatured during the process. The extent of protein denaturation was found to increase with the applied square wave voltage and electrolyte concentration. The solution temperature at the end of a theta tip was measured ...[more]