Unknown

Dataset Information

0

An Extended Approach to Quantify Triacylglycerol in Microalgae by Characteristic Fatty Acids.


ABSTRACT: Microalgae represent a third generation biofuel feedstock due to their high triacylglycerol (TAG) content under adverse environmental conditions. Microalgal TAG resides in a single cell and serves as a lipid class mixed with complicated compositions. We previously showed that TAG possessed characteristic fatty acids (CFAs) for quantification and was linearly correlated with the relative abundance of CFA within certain limits in microalgae. Here, we defined the application range of the linear correlation between TAG and CFA in the oleaginous microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum. In addition, TAG quantification was further expanded to a wide range of levels and the absolute amounts of saturated or monounsaturated CFAs, 16:0 and 18:1n9 of C. reinhardtii and 16:0 and 16:1n7 of P. tricornutum, instead of polyunsaturated CFAs, were verified to be linearly correlated to TAG levels throughout the entire period of nitrogen stress. This approach utilizes a single fatty acid to quantify TAG mixtures, and is rapid, simple and precise, which provides a useful tool for monitoring TAG accumulation of distinct microalgal species and facilitating high-throughput mutant screening for microalgae.

SUBMITTER: Yang M 

PROVIDER: S-EPMC5693890 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

altmetric image

Publications

An Extended Approach to Quantify Triacylglycerol in Microalgae by Characteristic Fatty Acids.

Yang Miao M   Yang Miao M   Fan Yan Y   Wu Pei-Chun PC   Chu Ya-Dong YD   Shen Pei-Li PL   Xue Song S   Chi Zhan-You ZY  

Frontiers in plant science 20171113


Microalgae represent a third generation biofuel feedstock due to their high triacylglycerol (TAG) content under adverse environmental conditions. Microalgal TAG resides in a single cell and serves as a lipid class mixed with complicated compositions. We previously showed that TAG possessed characteristic fatty acids (CFAs) for quantification and was linearly correlated with the relative abundance of CFA within certain limits in microalgae. Here, we defined the application range of the linear cor  ...[more]

Similar Datasets

| S-EPMC4761805 | biostudies-literature
2012-02-21 | E-GEOD-31901 | biostudies-arrayexpress
2012-02-21 | GSE31901 | GEO
| S-EPMC3196241 | biostudies-literature
| S-EPMC6077587 | biostudies-literature
| S-EPMC4206773 | biostudies-literature
| S-EPMC4023412 | biostudies-literature
| S-EPMC6016142 | biostudies-literature
| S-EPMC7360907 | biostudies-literature
| S-EPMC3478515 | biostudies-literature