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Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome.

ABSTRACT: We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753).

SUBMITTER: Amelia TSM 

PROVIDER: S-EPMC5694960 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Data on partial polyhydroxyalkanoate synthase genes <i>(phaC)</i> mined from <i>Aaptos aaptos</i> marine sponge-associated bacteria metagenome.

Amelia Tan Suet May TSM   Amirul Al-Ashraf Abdullah AA   Bhubalan Kesaven K  

Data in brief 20171108

We report data associated with the identification of three polyhydroxyalkanoate synthase genes <i>(phaC)</i> isolated from the marine bacteria metagenome of <i>Aaptos aaptos</i> marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II <i>phaC  ...[more]

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