The ssrA-Tag Facilitated Degradation of an Integral Membrane Protein.
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ABSTRACT:
SUBMITTER: Chai Q
PROVIDER: S-EPMC5695868 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Publications
The ssrA-Tag Facilitated Degradation of an Integral Membrane Protein.
Chai Qian Q Wang Zhaoshuai Z Webb Stacy R SR Dutch Rebecca E RE Wei Yinan Y
Biochemistry 20160418 16
ATP-dependent degradation plays a critical role in the quality control and recycling of proteins in cells. However, complete degradation of membrane proteins by ATP-dependent proteases in bacteria is not well-studied. We discovered that the degradation of a multidomain and multispan integral membrane protein AcrB could be facilitated by the introduction of a ssrA-tag at the C-terminus of the protein sequence and demonstrated that the cytoplasmic unfoldase-protease complex ClpXP was involved in t ...[more]
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