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Open-source do-it-yourself multi-color fluorescence smartphone microscopy.


ABSTRACT: Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of-care diagnostics. Current fluorescence smartphone microscope setups require precise illumination and imaging alignment which altogether limit its broad adoption. We report a multi-color fluorescence smartphone microscope with a single contact lens-like add-on lens and slide-launched total-internal-reflection guided illumination for three common tasks in investigative fluorescence microscopy: autofluorescence, fluorescent stains, and immunofluorescence. The open-source, simple and cost-effective design has the potential for do-it-yourself fluorescence smartphone microscopy.

SUBMITTER: Sung Y 

PROVIDER: S-EPMC5695954 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Open-source do-it-yourself multi-color fluorescence smartphone microscopy.

Sung Yulung Y   Campa Fernando F   Shih Wei-Chuan WC  

Biomedical optics express 20171019 11


Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of-care diagnostics. Current fluorescence smartphone microscope setups require precise illumination and imaging alignment which altogether limit its broad adoption. We report a multi-color fluorescence smartphone microscope with a single contact lens-l  ...[more]

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