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ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.


ABSTRACT: The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ?chrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.

SUBMITTER: Baaziz H 

PROVIDER: S-EPMC5699817 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.

Baaziz Hiba H   Gambari Cyril C   Boyeldieu Anne A   Ali Chaouche Amine A   Alatou Radia R   Méjean Vincent V   Jourlin-Castelli Cécile C   Fons Michel M  

PloS one 20171122 11


The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E.  ...[more]

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