Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
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ABSTRACT:
SUBMITTER: Roversi P
PROVIDER: S-EPMC5700055 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Roversi Pietro P Johnson Steven S Preston Stephen G SG Nunn Miles A MA Paesen Guido C GC Austyn Jonathan M JM Nuttall Patricia A PA Lea Susan M SM
Scientific reports 20171122 1
Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology ...[more]
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