Dataset Information

Transcription factor NF-?B is modulated by symbiotic status in a sea anemone model of cnidarian bleaching.

ABSTRACT: Transcription factor NF-?B plays a central role in immunity from fruit flies to humans, and NF-?B activity is altered in many human diseases. To investigate a role for NF-?B in immunity and disease on a broader evolutionary scale we have characterized NF-?B in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., "bleaching"). We show that the DNA-binding site specificity of Aiptasia NF-?B is similar to NF-?B proteins from a broad expanse of organisms. Analyses of NF-?B and I?B kinase proteins from Aiptasia suggest that non-canonical NF-?B processing is an evolutionarily ancient pathway, which can be reconstituted in human cells. In Aiptasia, NF-?B protein levels, DNA-binding activity, and tissue expression increase when loss of the algal symbiont Symbiodinium is induced by heat or chemical treatment. Kinetic analysis of NF-?B levels following loss of symbiosis show that NF-?B levels increase only after Symbiodinium is cleared. Moreover, introduction of Symbiodinium into naïve Aiptasia larvae results in a decrease in NF-?B expression. Our results suggest that Symbiodinium suppresses NF-?B in order to enable establishment of symbiosis in Aiptasia. These results are the first to demonstrate a link between changes in the conserved immune regulatory protein NF-?B and cnidarian symbiotic status.

SUBMITTER: Mansfield KM

PROVIDER: S-EPMC5700166 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching.

Mansfield Katelyn M KM Carter Nicole M NM Nguyen Linda L Cleves Phillip A PA Alshanbayeva Anar A Williams Leah M LM Crowder Camerron C Penvose Ashley R AR Finnerty John R JR Weis Virginia M VM Siggers Trevor W TW Gilmore Thomas D TD

Scientific reports 20171122 1

Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., "bleaching"). We show that the DNA-binding site specificity of Aiptasia NF-κB is similar to NF-κB proteins from a broad expanse o ...[more]

PMID: 29167511

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Dataset Information

Transcription factor NF-?B is modulated by symbiotic status in a sea anemone model of cnidarian bleaching.

Publications

Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching.

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