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Role of Alanine Racemase Mutations in Mycobacterium tuberculosis d-Cycloserine Resistance.

ABSTRACT: A screening of more than 1,500 drug-resistant strains of Mycobacterium tuberculosis revealed evolutionary patterns characteristic of positive selection for three alanine racemase (Alr) mutations. We investigated these mutations using molecular modeling, in vitro MIC testing, as well as direct measurements of enzymatic activity, which demonstrated that these mutations likely confer resistance to d-cycloserine.

SUBMITTER: Nakatani Y 

PROVIDER: S-EPMC5700341 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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A screening of more than 1,500 drug-resistant strains of <i>Mycobacterium tuberculosis</i> revealed evolutionary patterns characteristic of positive selection for three alanine racemase (Alr) mutations. We investigated these mutations using molecular modeling, <i>in vitro</i> MIC testing, as well as direct measurements of enzymatic activity, which demonstrated that these mutations likely confer resistance to d-cycloserine. ...[more]

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