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Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening.

ABSTRACT: This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10?µL of biofluid, with limits of quantitation in the low/sub ng·mL-1 level attained within 7?minutes.

SUBMITTER: Gomez-Rios GA

PROVIDER: S-EPMC5701014 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening.

Gómez-Ríos Germán Augusto GA Tascon Marcos M Reyes-Garcés Nathaly N Boyacı Ezel E Poole Justen J Pawliszyn Janusz J

Scientific reports 20171123 1

This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Divers ...[more]

PMID: 29170449

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