Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates.
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ABSTRACT:
SUBMITTER: Gan J
PROVIDER: S-EPMC5702260 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates.
Gan Jinrui J Ben-Nissan Gili G Arkind Galina G Tarnavsky Mark M Trudeau Devin D Noda Garcia Lianet L Tawfik Dan S DS Sharon Michal M
Analytical chemistry 20170405 8
Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification. ...[more]
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