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Growth arrest and a persister state enable resistance to osmotic shock and facilitate dissemination of Vibrio cholerae.

ABSTRACT: Vibrio cholerae is a water-borne bacterial pathogen and causative agent of cholera. Although V. cholerae is a halophile, it can survive in fresh water, and this has a major role in cholera epidemics through consumption of contaminated water and subsequent fecal-oral spread. After dissemination from humans back into fresh water, V. cholerae encounters limited nutrient availability and an abrupt drop in conductivity but little is known about how V. cholerae adapts to, and survives in this environment. In this work, by abolishing or altering the expression of V. cholerae genes in a high-throughput manner, we observed that many osmotic shock tolerant mutants exhibited slowed or arrested growth, and/or generated a higher proportion of persister cells. In addition, we show that growth-arrested V. cholerae, including a persister subpopulation, are generated during infection of the intestinal tract and together allow for the successful dissemination to fresh water. Our results suggest that growth-arrested and persister subpopulations enable survival of V. cholerae upon shedding to the aquatic environment.

SUBMITTER: Silva-Valenzuela CA

PROVIDER: S-EPMC5702728 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Growth arrest and a persister state enable resistance to osmotic shock and facilitate dissemination of Vibrio cholerae.

Silva-Valenzuela Cecilia A CA Lazinski David W DW Kahne Shoshanna C SC Nguyen Y Y Molina-Quiroz Roberto C RC Camilli Andrew A

The ISME journal 20170725 12

Vibrio cholerae is a water-borne bacterial pathogen and causative agent of cholera. Although V. cholerae is a halophile, it can survive in fresh water, and this has a major role in cholera epidemics through consumption of contaminated water and subsequent fecal-oral spread. After dissemination from humans back into fresh water, V. cholerae encounters limited nutrient availability and an abrupt drop in conductivity but little is known about how V. cholerae adapts to, and survives in this environm ...[more]

PMID: 28742070

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Dataset Information

Growth arrest and a persister state enable resistance to osmotic shock and facilitate dissemination of Vibrio cholerae.

Publications

Growth arrest and a persister state enable resistance to osmotic shock and facilitate dissemination of Vibrio cholerae.

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