RNA-Seq Analysis of Plant Maturity in Crested Wheatgrass (Agropyron cristatum L.).

ABSTRACT: Crested wheatgrass (Agropyron cristatum L.) breeding programs aim to develop later maturing cultivars for extending early spring grazing in Western Canada. Plant maturity is a complex genetic trait, and little is known about genes associated with late maturity in this species. An attempt was made using RNA-Seq to profile the transcriptome of crested wheatgrass maturity and to analyze differentially expressed genes (DEGs) between early and late maturing lines. Three cDNA libraries for each line were generated by sampling leaves at the stem elongation stage, spikes at the boot and anthesis stages. A total of 75,218,230 and 74,015,092 clean sequence reads were obtained for early and late maturing lines, respectively. De novo assembly of all sequence reads generated 401,587 transcripts

SUBMITTER: Zeng F 

PROVIDER: S-EPMC5704204 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature