Unknown

Dataset Information

0

Identification of the Caprine Keratin-Associated Protein 20-2 (KAP20-2) Gene and Its Effect on Cashmere Traits.


ABSTRACT: The gene encoding the high glycine/tyrosine keratin-associated protein 20-2 (KAP20-2) gene has been described in humans, but has not been identified in any livestock species. A search for similar sequences in the caprine genome using the human KAP20-2 gene (KRTAP20-2) revealed a homologous sequence on chromosome 1. Three different banding patterns representing distinct sequences (A-C) in Longdong cashmere goats were identified using polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis. These sequences shared high sequence similarity with the human and mouse KRTAP20-2 sequences, suggesting that A-C are caprine variants of the human and mouse genes. Four single nucleotide polymorphisms (SNPs) were identified, and three of them were non-synonymous. KRTAP20-2 was found to be expressed in secondary hair follicles, but not in heart, liver, lung, kidney, spleen, or longissimus dorsi muscle. The presence of A was associated with increased cashmere fibre weight, while the presence of B was associated with a decrease in cashmere fibre weight and curly fibre length. Goats with genotype AA had a higher cashmere fibre weight and a higher curly fibre length than those with genotypes AB or BB. These results indicate that caprine KRTAP20-2 variation may have value as a genetic marker for improving cashmere fibre weight.

SUBMITTER: Wang J 

PROVIDER: S-EPMC5704241 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification of the Caprine Keratin-Associated Protein 20-2 (KAP20-2) Gene and Its Effect on Cashmere Traits.

Wang Jiqing J   Che Longjie L   Hickford Jon G H JGH   Zhou Huitong H   Hao Zhiyun Z   Luo Yuzhu Y   Hu Jiang J   Liu Xiu X   Li Shaobin S  

Genes 20171117 11


The gene encoding the high glycine/tyrosine keratin-associated protein 20-2 (KAP20-2) gene has been described in humans, but has not been identified in any livestock species. A search for similar sequences in the caprine genome using the human KAP20-2 gene (<i>KRTAP20-2</i>) revealed a homologous sequence on chromosome 1. Three different banding patterns representing distinct sequences (<i>A</i>-<i>C</i>) in Longdong cashmere goats were identified using polymerase chain reaction-single stranded  ...[more]

Similar Datasets

| S-EPMC8442941 | biostudies-literature
| S-EPMC7463587 | biostudies-literature
| S-EPMC6852866 | biostudies-literature
| S-EPMC10487131 | biostudies-literature
| S-EPMC7074440 | biostudies-literature
| S-EPMC5295022 | biostudies-literature
| S-EPMC8657448 | biostudies-literature
| S-EPMC8143586 | biostudies-literature
| S-EPMC5615358 | biostudies-literature
| S-EPMC6680380 | biostudies-literature