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RAC3 influences the chemoresistance of colon cancer cells through autophagy and apoptosis inhibition.


ABSTRACT: Background:RAC3 coactivator overexpression has been implicated in tumorigenesis, contributing to inhibition of apoptosis and autophagy. Both mechanisms are involved in resistance to treatment with chemotherapeutic agents. The aim of this study was to investigate its role in chemoresistance of colorectal cancer. Methods:The sensitivity to 5-fluorouracil and oxaliplatin in colon cancer cells HT-29, HCT 116 and Lovo cell lines, expressing high or low natural levels of RAC3, was investigated using viability assays. Results:In HCT 116 cells, we found that although 5-fluorouracil was a poor inducer of apoptosis, autophagy was strongly induced, while oxaliplatin has shown a similar ability to induce both of them. However, in HCT 116 cells expressing a short hairpin RNA for RAC3, we found an increased sensitivity to both drugs if it is compared with control cells. 5-Fluorouracil and oxaliplatin treatment lead to an enhanced caspase 3-dependent apoptosis and produce an increase of autophagy. In addition, both process have shown to be trigged faster than in control cells, starting earlier after stimulation. Conclusions:Our results suggest that RAC3 expression levels influence the sensitivity to chemotherapeutic drugs. Therefore, the knowledge of RAC3 expression levels in tumoral samples could be an important contribution to design new improved therapeutic strategies in the future.

SUBMITTER: Rubio MF 

PROVIDER: S-EPMC5706160 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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RAC3 influences the chemoresistance of colon cancer cells through autophagy and apoptosis inhibition.

Rubio María Fernanda MF   Lira María Cecilia MC   Rosa Francisco Damián FD   Sambresqui Adrían Dario AD   Salazar Güemes María Cecilia MC   Costas Mónica Alejandra MA   Costas Mónica Alejandra MA  

Cancer cell international 20171128


<h4>Background</h4>RAC3 coactivator overexpression has been implicated in tumorigenesis, contributing to inhibition of apoptosis and autophagy. Both mechanisms are involved in resistance to treatment with chemotherapeutic agents. The aim of this study was to investigate its role in chemoresistance of colorectal cancer.<h4>Methods</h4>The sensitivity to 5-fluorouracil and oxaliplatin in colon cancer cells HT-29, HCT 116 and Lovo cell lines, expressing high or low natural levels of RAC3, was inves  ...[more]

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