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Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading.


ABSTRACT: Bovine herpesvirus 1 (BoHV-1) UL21 is a tegument protein thought to be indispensable for efficient viral growth but its precise function in BoHV-1 is currently unknown. To determine the function of UL21 in BoHV-1 replication, we constructed a mutant virus bearing a UL21 deletion (vBoHV-1-?UL21) and its revertant virus, vBoHV-1-?UL21R, in which the UL21 gene was restored using a bacterial artificial chromosome system. The replication of vBoHV-1-?UL21 was 1,000-fold lower and its plaque size was 85% smaller than those of the wild-type virus (BoHV-1). An ultrastructural analysis showed that deletion of UL21 led to an un-enveloped capsid accumulation in the cytoplasm, whereas nucleocapsid egress was not impaired, suggesting that UL21 is critical for secondary envelopment in BoHV-1. Co-immunoprecipitation assays revealed that HA-tagged UL21 pulled down UL16, suggesting that these two proteins form a complex, and this was further confirmed by a co-immunofluorescence assay. Taken together, these data provide evidence that UL21 plays critical roles in BoHV-1 secondary envelopment, and UL16 is likely to be involved in these activities.

SUBMITTER: Shahin F 

PROVIDER: S-EPMC5706888 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Bovine herpesvirus 1 tegument protein <i>UL21</i> plays critical roles in viral secondary envelopment and cell-to-cell spreading.

Shahin Farzana F   Raza Sohail S   Yang Kui K   Hu Changmin C   Chen Yingyu Y   Chen Huanchun H   Guo Aizhen A  

Oncotarget 20171010 55


<i>Bovine herpesvirus 1</i> (BoHV-1) <i>UL21</i> is a tegument protein thought to be indispensable for efficient viral growth but its precise function in BoHV-1 is currently unknown. To determine the function of <i>UL21</i> in BoHV-1 replication, we constructed a mutant virus bearing a <i>UL21</i> deletion (vBoHV-1-∆UL21) and its revertant virus, vBoHV-1-∆UL21R, in which the <i>UL21</i> gene was restored using a bacterial artificial chromosome system. The replication of vBoHV-1-∆UL21 was 1,000-f  ...[more]

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