Ontology highlight
ABSTRACT:
SUBMITTER: Singh RR
PROVIDER: S-EPMC5707199 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Singh Rajesh R RR Bains Ashish A Patel Keyur P KP Rahimi Hamed H Barkoh Bedia A BA Paladugu Abhaya A Bisrat Tigist T Ravandi-Kashani Farhad F Cortes Jorge E JE Kantarjian Hagop M HM Medeiros L Jeffrey LJ Luthra Rajyalakshmi R
The Journal of molecular diagnostics : JMD 20120527 4
DNA methyltransferase 3A (DNMT3A) is mutated in a subset of de novo acute myeloid leukemia patients and is associated with poor overall and event-free survival. Because routine Sanger sequencing of the 23 DNMT3A exons is impractical in clinical laboratories, we developed a high-throughput method using high-resolution melting (HRM) analysis, which identifies sequence variants by detecting subtle changes in the melting patterns of mutant DNA in comparison with WT sequences. DNA from 104 acute myel ...[more]