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Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy.

ABSTRACT: A photocrosslinkable protein G variant was used as an adapter protein to covalently and site-specifically conjugate an antibody and an Fc-fusion protein to an oligonucleotide. This modular approach enables straightforward decoration of DNA nanostructures with complex native proteins while retaining their innate binding affinity, allowing precise control over the nanoscale spatial organization of such proteins for in vitro and in vivo biomedical applications.

SUBMITTER: Rosier BJHM

PROVIDER: S-EPMC5708335 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy.

Rosier Bas J H M BJHM Cremers Glenn A O GAO Engelen Wouter W Merkx Maarten M Brunsveld Luc L de Greef Tom F A TFA

Chemical communications (Cambridge, England) 20170601 53

A photocrosslinkable protein G variant was used as an adapter protein to covalently and site-specifically conjugate an antibody and an Fc-fusion protein to an oligonucleotide. This modular approach enables straightforward decoration of DNA nanostructures with complex native proteins while retaining their innate binding affinity, allowing precise control over the nanoscale spatial organization of such proteins for in vitro and in vivo biomedical applications. ...[more]

PMID: 28617516

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