Improved Detection of Cytokines Produced by Invariant NKT Cells.
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ABSTRACT:
SUBMITTER: Sag D
PROVIDER: S-EPMC5709402 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
Improved Detection of Cytokines Produced by Invariant NKT Cells.
Sag Duygu D Özkan Müge M Kronenberg Mitchell M Wingender Gerhard G
Scientific reports 20171130 1
Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detectio ...[more]
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