A robust cell culture system supporting the complete life cycle of hepatitis B virus.
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ABSTRACT:
SUBMITTER: Michailidis E
PROVIDER: S-EPMC5709435 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
A robust cell culture system supporting the complete life cycle of hepatitis B virus.
Michailidis Eleftherios E Pabon Jonathan J Xiang Kuanhui K Park Paul P Ramanan Vyas V Hoffmann Hans-Heinrich HH Schneider William M WM Bhatia Sangeeta N SN de Jong Ype P YP Shlomai Amir A Rice Charles M CM
Scientific reports 20171130 1
The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the hepatitis B virus (HBV) receptor enabled researchers to create hepatoma cell lines susceptible to HBV infection. Infection in current systems, however, is inefficient and virus fails to spread. Infection efficiency is enhanced by treating cells with polyethylene glycol 8000 (PEG) during infection. However, this alone does not promote virus spread. Here we show that maintaining PEG in culture medium increases the rate o ...[more]
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