Dataset Information

Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma.

ABSTRACT: As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

SUBMITTER: Bowden JA

PROVIDER: S-EPMC5711491 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma.

Bowden John A JA Heckert Alan A Ulmer Candice Z CZ Jones Christina M CM Koelmel Jeremy P JP Abdullah Laila L Ahonen Linda L Alnouti Yazen Y Armando Aaron M AM Asara John M JM Bamba Takeshi T Barr John R JR Bergquist Jonas J Borchers Christoph H CH Brandsma Joost J Breitkopf Susanne B SB Cajka Tomas T Cazenave-Gassiot Amaury A Checa Antonio A Cinel Michelle A MA Colas Romain A RA Cremers Serge S Dennis Edward A EA Evans James E JE Fauland Alexander A Fiehn Oliver O Gardner Michael S MS Garrett Timothy J TJ Gotlinger Katherine H KH Han Jun J Huang Yingying Y Neo Aveline Huipeng AH Hyötyläinen Tuulia T Izumi Yoshihiro Y Jiang Hongfeng H Jiang Houli H Jiang Jiang J Kachman Maureen M Kiyonami Reiko R Klavins Kristaps K Klose Christian C Köfeler Harald C HC Kolmert Johan J Koal Therese T Koster Grielof G Kuklenyik Zsuzsanna Z Kurland Irwin J IJ Leadley Michael M Lin Karen K Maddipati Krishna Rao KR McDougall Danielle D Meikle Peter J PJ Mellett Natalie A NA Monnin Cian C Moseley M Arthur MA Nandakumar Renu R Oresic Matej M Patterson Rainey R Peake David D Pierce Jason S JS Post Martin M Postle Anthony D AD Pugh Rebecca R Qiu Yunping Y Quehenberger Oswald O Ramrup Parsram P Rees Jon J Rembiesa Barbara B Reynaud Denis D Roth Mary R MR Sales Susanne S Schuhmann Kai K Schwartzman Michal Laniado ML Serhan Charles N CN Shevchenko Andrej A Somerville Stephen E SE St John-Williams Lisa L Surma Michal A MA Takeda Hiroaki H Thakare Rhishikesh R Thompson J Will JW Torta Federico F Triebl Alexander A Trötzmüller Martin M Ubhayasekera S J Kumari SJK Vuckovic Dajana D Weir Jacquelyn M JM Welti Ruth R Wenk Markus R MR Wheelock Craig E CE Yao Libin L Yuan Min M Zhao Xueqing Heather XH Zhou Senlin S

Journal of lipid research 20171006 12

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus l ...[more]

PMID: 28986437

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Project description:Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope labeling, optimize collision energy and generate in silico spectral libraries. We validate LipidCreator assays computationally and analytically and prove that it is capable to generate large targeted experiments to analyze blood and to dissect lipid-signaling pathways such as in human platelets. In MTBLS1375, to validate tissue-specific transitions which we generated with LipidCreator, we conducted quantitative lipidomics experiments monitoring 433 lipids in the plasma of 21 healthy Asian human subjects. For a subset of 50 individual lipid levels the concentrations were compared to a recently conducted ring trial monitoring the lipid content of a reference material obtained from a mixed American population (NIST SRM 1950). The rationale behind this was to see if the creation of a list including internal standard and endogenous lipids lead to similar quantitative results as the ring trial experiment. Studies linked to this manuscript include;<a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1333' rel='noopener noreferrer' target='_blank'>MTBLS1333; LipidCreator: Collision Energy Optimization and Fragment Intensity Prediction for Lipid Mediators - Thermo QExactive HF</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1334' rel='noopener noreferrer' target='_blank'>MTBLS1334; LipidCreator: Collision Energy Optimization and Fragment Intensity Prediction for Lipid Mediators - Agilent QTof</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1369' rel='noopener noreferrer' target='_blank'>MTBLS1369; LipidCreator: Platelet isolation and stimulation - Targeted Phospho- and Glycerolipid Profiling</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1375' rel='noopener noreferrer' target='_blank'>MTBLS1375; LipidCreator: Targeted lipidomics analysis of Human Plasma samples and comparison with NIST SRM 1950 standard</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1376' rel='noopener noreferrer' target='_blank'>MTBLS1376; LipidCreator: Targeted lipidomics analysis of S. cerevisiae to determine true and false identification</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1381' rel='noopener noreferrer' target='_blank'>MTBLS1381; LipidCreator: Platelet isolation and stimulation - Targeted Lipid Mediator Profiling</a><a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS1382' rel='noopener noreferrer' target='_blank'>MTBLS1382; LipidCreator: Platelet isolation and stimulation - DIA Lipid Mediator Validation</a>

Dataset Information

Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma.

Publications

Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma.

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