Glutaraldehyde Cross-linking of HIV-1 Env Trimers Skews the Antibody Subclass Response in Mice.
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ABSTRACT:
SUBMITTER: Soldemo M
PROVIDER: S-EPMC5711779 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
Glutaraldehyde Cross-linking of HIV-1 Env Trimers Skews the Antibody Subclass Response in Mice.
Soldemo Martina M Àdori Monika M Stark Julian M JM Feng Yu Y Tran Karen K Wilson Richard R Yang Lifei L Guenaga Javier J Wyatt Richard T RT Karlsson Hedestam Gunilla B GB
Frontiers in immunology 20171127
Well-ordered soluble HIV-1 envelope glycoprotein (Env) spike mimetics such as Native Flexibly Linked (NFL) trimers display high homogeneity, desired antigenicity, and high <i>in vitro</i> stability compared to previous generation soluble HIV-1 Env trimers. Glutaraldehyde (GLA) cross-linking was shown to further increase the thermostability of clade C 16055 NFL trimers and enhance the induction of tier 2 autologous neutralizing antibodies in guinea pigs. Here, we investigated if GLA fixation affe ...[more]
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