Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures.
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ABSTRACT:
SUBMITTER: Santos RB
PROVIDER: S-EPMC5711867 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
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Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures.
Santos Rita B RB Pires Ana Sofia AS Abranches Rita R
Scientific reports 20171201 1
Plant cell cultures are an attractive platform for the production of recombinant proteins. A major drawback, hindering the establishment of plant cell suspensions as an industrial platform, is the low product yield obtained thus far. Histone acetylation is associated with increased transcription levels, therefore it is expected that the use of histone deacetylase inhibitors would result in an increase in mRNA and protein levels. Here, this hypothesis was tested by adding a histone deacetylase in ...[more]
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