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Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.

ABSTRACT: The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

SUBMITTER: Dai Q 

PROVIDER: S-EPMC5712428 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.

Dai Qing Q   Moshitch-Moshkovitz Sharon S   Han Dali D   Kol Nitzan N   Amariglio Ninette N   Rechavi Gideon G   Dominissini Dan D   He Chuan C  

Nature methods 20170515 7

The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles. ...[more]

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