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Functional Characterization of Waterlogging and Heat Stresses Tolerance Gene Pyruvate decarboxylase 2 from Actinidia deliciosa.


ABSTRACT: A previous report showed that both Pyruvatedecarboxylase (PDC) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit AdPDC1 gene was required during waterlogging, but might not be required during other environmental stresses. Here, the function of another PDC gene, named AdPDC2, was analyzed. The expression of the AdPDC2 gene was determined using qRT-PCR, and the results showed that the expression levels of AdPDC2 in the reproductive organs were much higher than those in the nutritive organs. Waterlogging, NaCl, and heat could induce the expression of AdPDC2. Overexpression of kiwifruit AdPDC2 in transgenic Arabidopsis enhanced resistance to waterlogging and heat stresses in five-week-old seedlings, but could not enhance resistance to NaCl and mannitol stresses at the seed germination stage and in early seedlings. These results suggested that the kiwifruit AdPDC2 gene may play an important role in waterlogging resistance and heat stresses in kiwifruit.

SUBMITTER: Luo HT 

PROVIDER: S-EPMC5713346 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Functional Characterization of Waterlogging and Heat Stresses Tolerance Gene Pyruvate decarboxylase 2 from Actinidia deliciosa.

Luo Hui-Ting HT   Zhang Ji-Yu JY   Wang Gang G   Jia Zhan-Hui ZH   Huang Sheng-Nan SN   Wang Tao T   Guo Zhong-Ren ZR  

International journal of molecular sciences 20171109 11


A previous report showed that both <i>Pyruvate</i><i>decarboxylase</i> (<i>PDC</i>) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit <i>AdPDC1</i> gene was required during waterlogging, but might not be required during other environmental stresses. Here, the function of another <i>PDC</i> gene, named <i>AdPDC2</i>, was analyzed. The expression of the <i>AdPDC2</i> gene was determined using qRT-PCR, and the  ...[more]

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