Unknown

Dataset Information

0

Approaches to ab initio molecular replacement of ?-helical transmembrane proteins.


ABSTRACT: ?-Helical transmembrane proteins are a ubiquitous and important class of proteins, but present difficulties for crystallographic structure solution. Here, the effectiveness of the AMPLE molecular replacement pipeline in solving ?-helical transmembrane-protein structures is assessed using a small library of eight ideal helices, as well as search models derived from ab initio models generated both with and without evolutionary contact information. The ideal helices prove to be surprisingly effective at solving higher resolution structures, but ab initio-derived search models are able to solve structures that could not be solved with the ideal helices. The addition of evolutionary contact information results in a marked improvement in the modelling and makes additional solutions possible.

SUBMITTER: Thomas JMH 

PROVIDER: S-EPMC5713875 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Approaches to ab initio molecular replacement of α-helical transmembrane proteins.

Thomas Jens M H JMH   Simkovic Felix F   Keegan Ronan R   Mayans Olga O   Zhang Chengxin C   Zhang Yang Y   Rigden Daniel J DJ  

Acta crystallographica. Section D, Structural biology 20171122 Pt 12


α-Helical transmembrane proteins are a ubiquitous and important class of proteins, but present difficulties for crystallographic structure solution. Here, the effectiveness of the AMPLE molecular replacement pipeline in solving α-helical transmembrane-protein structures is assessed using a small library of eight ideal helices, as well as search models derived from ab initio models generated both with and without evolutionary contact information. The ideal helices prove to be surprisingly effecti  ...[more]

Similar Datasets

| S-EPMC2374033 | biostudies-literature
| S-EPMC2533517 | biostudies-literature
| S-EPMC2808916 | biostudies-literature
| S-EPMC8764525 | biostudies-literature
| S-EPMC2739800 | biostudies-literature
| S-EPMC4321487 | biostudies-literature
| S-EPMC9062415 | biostudies-literature
| S-EPMC1514348 | biostudies-literature
| S-EPMC2597722 | biostudies-literature
| S-EPMC2876129 | biostudies-literature