Ontology highlight
ABSTRACT:
SUBMITTER: Mercier E
PROVIDER: S-EPMC5714171 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Mercier Evan E Holtkamp Wolf W Rodnina Marina V MV Wintermeyer Wolfgang W
Nucleic acids research 20171101 20
The bacterial signal recognition particle (SRP) is part of the machinery that targets ribosomes synthesizing membrane proteins to membrane-embedded translocons co-translationally. Recognition of nascent membrane proteins occurs by virtue of a hydrophobic signal-anchor sequence (SAS) contained in the nascent chain, usually at the N terminus. Here we use fluorescence-based stopped-flow to monitor SRP-ribosome interactions with actively translating ribosomes while an SRP substrate is synthesized an ...[more]