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Link between Affinity and Cu(II) Binding Sites to Amyloid-? Peptides Evaluated by a New Water-Soluble UV-Visible Ratiometric Dye with a Moderate Cu(II) Affinity.


ABSTRACT: Being able to easily determine the Cu(II) affinity for biomolecules of moderate affinity is important. Such biomolecules include amyloidogenic peptides, such as the well-known amyloid-? peptide involved in Alzheimer's disease. Here, we report the synthesis of a new water-soluble ratiometric Cu(II) dye with a moderate affinity (109 M-1 at pH 7.1) and the characterizations of the Cu(II) corresponding complex by X-ray crystallography, EPR, and XAS spectroscopic methods. UV-vis competition was performed on the A? peptide as well as on a wide series of modified peptides, leading to an affinity value of 1.6 × 109 M-1 at pH 7.1 for the A? peptide and to a coordination model for the Cu(II) site within the A? peptide that agrees with the one mostly accepted currently.

SUBMITTER: Conte-Daban A 

PROVIDER: S-EPMC5714188 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Link between Affinity and Cu(II) Binding Sites to Amyloid-β Peptides Evaluated by a New Water-Soluble UV-Visible Ratiometric Dye with a Moderate Cu(II) Affinity.

Conte-Daban Amandine A   Borghesani Valentina V   Sayen Stéphanie S   Guillon Emmanuel E   Journaux Yves Y   Gontard Geoffrey G   Lisnard Laurent L   Hureau Christelle C  

Analytical chemistry 20170125 3


Being able to easily determine the Cu(II) affinity for biomolecules of moderate affinity is important. Such biomolecules include amyloidogenic peptides, such as the well-known amyloid-β peptide involved in Alzheimer's disease. Here, we report the synthesis of a new water-soluble ratiometric Cu(II) dye with a moderate affinity (10<sup>9</sup> M<sup>-1</sup> at pH 7.1) and the characterizations of the Cu(II) corresponding complex by X-ray crystallography, EPR, and XAS spectroscopic methods. UV-vis  ...[more]

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