Dexmedetomidine alleviates LPS-induced septic cardiomyopathy via the cholinergic anti-inflammatory pathway in mice.

ABSTRACT: This study was conducted to investigate the role of the cholinergic anti-inflammatory pathway in LPS-induced septic cardiomyopathy in mice. C57BL/6 mice were used to construct septic cardiomyopathy models. The optimal duration of lipopolysaccharide (LPS) treatment was determined by HE staining and TUNEL assay. Blank controls were intraperitoneally injected with saline and models were injected with LPS (10 mg/kg) (LPS), ?-bungarotoxin (BT-LPS), BT and dexmedetomidine (BT-DEX-LPS). The pathological examinations were performed on HE- stained myocardium tissues, apoptosis was determined using TUNEL assay, mRNA expression of NF-?B p65, Caspase-3, Caspase-8, Bcl-2, Bax, p53 and ?7nACh was quantified using qRT-PCR, protein levels of IL-6, IL-1?, TNF-? and phosphorylated STAT3 (p-STAT3) were analyzed using Western blot analysis. HE staining and TUNEL assays showed that the optimal LPS treatment time for septic cardiomyopathy induction was 16 h. Compared with the blank control, mice in LPS group had significantly higher apoptosis, while DEX and BT reduced apoptosis when they were used separately and increased apoptosis when they were used jointly. In the LPS-treated mice, the levels of NF-?b p65, Caspase-3, Caspase-8, Bax, p53, IL-6, IL-1?, TNF-? and p-STAT3 were significantly increased, while ?7nAChR level was decreased significantly (P < 0.01); DEX alone had no impact on the expression of these proteins but significantly up-regulated the expression of these genes except ?7nAChR when used jointly with BT (P < 0.01). It is clear that DEX can alleviate heart injury, while ?7nAChR-specific blocker BT is antagonistic against the anti-inflammatory effect of DEX on sepsis in mice.

SUBMITTER: Kong W 

PROVIDER: S-EPMC5714788 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature