Ontology highlight
ABSTRACT:
SUBMITTER: Esadze A
PROVIDER: S-EPMC5716149 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Esadze Alexandre A Rodriguez Gaddiel G Weiser Brian P BP Cole Philip A PA Stivers James T JT
Nucleic acids research 20171201 21
DNA 'sliding' by human repair enzymes is considered to be important for DNA damage detection. Here, we transfected uracil-containing DNA duplexes into human cells and measured the probability that nuclear human uracil DNA glycosylase (hUNG2) excised two uracil lesions spaced 10-80 bp apart in a single encounter without escaping the micro-volume containing the target sites. The two-site transfer probabilities were 100% and 54% at a 10 and 40 bp spacing, but dropped to only 10% at 80 bp. Enzyme tr ...[more]