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The nucleoid as a scaffold for the assembly of bacterial signaling complexes.

ABSTRACT: The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays.

SUBMITTER: Moine A

PROVIDER: S-EPMC5716589 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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The nucleoid as a scaffold for the assembly of bacterial signaling complexes.

Moine Audrey A Espinosa Leon L Martineau Eugenie E Yaikhomba Mutum M Jazleena P J PJ Byrne Deborah D Biondi Emanuele G EG Notomista Eugenio E Brilli Matteo M Molle Virginie V Gayathri Pananghat P Mignot Tâm T Mauriello Emilia M F EMF

PLoS genetics 20171121 11

The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore cons ...[more]

PMID: 29161263

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