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Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.

ABSTRACT: Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.

SUBMITTER: Kim CK

PROVIDER: S-EPMC5717315 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.

Kim Christina K CK Yang Samuel J SJ Pichamoorthy Nandini N Young Noah P NP Kauvar Isaac I Jennings Joshua H JH Lerner Talia N TN Berndt Andre A Lee Soo Yeun SY Ramakrishnan Charu C Davidson Thomas J TJ Inoue Masatoshi M Bito Haruhiko H Deisseroth Karl K

Nature methods 20160215 4

Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, ...[more]

PMID: 26878381

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