Ontology highlight
ABSTRACT:
SUBMITTER: Adikusuma F
PROVIDER: S-EPMC5718404 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Adikusuma Fatwa F Pfitzner Chandran C Thomas Paul Quinton PQ
PloS one 20171206 12
CRISPR/Cas9 technology enables efficient, rapid and cost-effective targeted genomic modification in a wide variety of cellular contexts including cultured cells. Some applications such as generation of double knock-outs, large deletions and paired-nickase cleavage require simultaneous expression of two gRNAs. Although single plasmids that enable multiplex expression of gRNAs have been developed, these require multiple rounds of cloning and/or PCR for generation of the desired construct. Here, we ...[more]