Unknown

Dataset Information

0

Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.


ABSTRACT: Together, the three human rhinovirus (RV) species are the most frequent cause of the common cold. Because of their high similarity with other viral species of the genus Enterovirus, within the large family Picornaviridae, studies on RV infectious activities often offer a less pathogenic model for more aggressive enteroviruses, e.g. poliovirus or EV71. Picornaviruses enter via receptor mediated endocytosis and replicate in the cytosol. Most of them depend on functional F-actin, Rab proteins, and probably motor proteins. To assess the latter, we evaluated the role of myosin light chain kinase (MLCK) and two myosin V isoforms (Va and Vb) in RV-B14 infection. We report that ML-9, a very specific MLCK inhibitor, dramatically reduced RV-B14 entry. We also demonstrate that RV-B14 infection in cells expressing dominant-negative forms of myosin Va and Vb was impaired after virus entry. Using immunofluorescent localization and immunoprecipitation, we show that myosin Va co-localized with RV-B14 exclusively after viral entry (15?min post infection) and that myosin Vb was present in the clusters of newly synthesized RNA in infected cells. These clusters, observed at 180?min post infection, are reminiscent of replication sites. Taken together, these results identify myosin light chain kinase, myosin Va and myosin Vb as new players in RV-B14 infection that participate directly or indirectly in different stages of the viral cycle.

SUBMITTER: Real-Hohn A 

PROVIDER: S-EPMC5719429 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.

Real-Hohn Antonio A   Provance D William DW   Gonçalves Rafael Braga RB   Denani Caio Bidueira CB   de Oliveira Andréa Cheble AC   Salerno Verônica P VP   Oliveira Gomes Andre Marco AM  

Scientific reports 20171207 1


Together, the three human rhinovirus (RV) species are the most frequent cause of the common cold. Because of their high similarity with other viral species of the genus Enterovirus, within the large family Picornaviridae, studies on RV infectious activities often offer a less pathogenic model for more aggressive enteroviruses, e.g. poliovirus or EV71. Picornaviruses enter via receptor mediated endocytosis and replicate in the cytosol. Most of them depend on functional F-actin, Rab proteins, and  ...[more]

Similar Datasets

| S-EPMC7734653 | biostudies-literature
| S-EPMC9106349 | biostudies-literature
| S-EPMC5544331 | biostudies-literature
| S-EPMC7769881 | biostudies-literature
| S-EPMC2599868 | biostudies-other
| S-EPMC10107955 | biostudies-literature
| S-EPMC8365777 | biostudies-literature
| S-EPMC4192498 | biostudies-literature
| S-EPMC4407996 | biostudies-literature
| S-EPMC6408230 | biostudies-literature