Ontology highlight
ABSTRACT:
SUBMITTER: Mathews II
PROVIDER: S-EPMC5721673 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Mathews Irimpan I II Allison Kim K Robbins Thomas T Lyubimov Artem Y AY Uervirojnangkoorn Monarin M Brunger Axel T AT Khosla Chaitan C DeMirci Hasan H McPhillips Scott E SE Hollenbeck Michael M Soltis Michael M Cohen Aina E AE
Biochemistry 20170831 36
The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 Å using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of microcrystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS ...[more]