Ontology highlight
ABSTRACT:
SUBMITTER: Bryson DI
PROVIDER: S-EPMC5724969 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Bryson David I DI Fan Chenguang C Guo Li-Tao LT Miller Corwin C Söll Dieter D Liu David R DR
Nature chemical biology 20171016 12
Directed evolution of orthogonal aminoacyl-tRNA synthetases (AARSs) enables site-specific installation of noncanonical amino acids (ncAAs) into proteins. Traditional evolution techniques typically produce AARSs with greatly reduced activity and selectivity compared to their wild-type counterparts. We designed phage-assisted continuous evolution (PACE) selections to rapidly produce highly active and selective orthogonal AARSs through hundreds of generations of evolution. PACE of a chimeric Methan ...[more]