Ontology highlight
ABSTRACT:
SUBMITTER: Nestor G
PROVIDER: S-EPMC5725960 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Nestor Gustav G Anderson Taigh T Oscarson Stefan S Gronenborn Angela M AM
Journal of the American Chemical Society 20170421 17
NMR of a uniformly <sup>13</sup>C-labeled carbohydrate was used to elucidate the atomic details of a sugar-protein complex. The structure of the <sup>13</sup>C-labeled Manα(1-2)Manα(1-2)ManαOMe trisaccharide ligand, when bound to cyanovirin-N (CV-N), was characterized and revealed that in the complex the glycosidic linkage torsion angles between the two reducing-end mannoses are different from the free trisaccharide. Distances within the carbohydrate were employed for conformational analysis, an ...[more]