Unknown

Dataset Information

0

Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing.


ABSTRACT: We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9?Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.

SUBMITTER: Gorel A 

PROVIDER: S-EPMC5726314 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications


We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model syste  ...[more]

Similar Datasets

| S-EPMC4667287 | biostudies-literature
| S-EPMC4566134 | biostudies-literature
| S-EPMC3478121 | biostudies-literature
| S-EPMC9005607 | biostudies-literature
| S-EPMC5332129 | biostudies-literature
| S-EPMC5135358 | biostudies-literature
| S-EPMC3438231 | biostudies-literature
| S-EPMC6385504 | biostudies-literature
| S-EPMC3902108 | biostudies-literature
| S-EPMC4909539 | biostudies-literature