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Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices.

ABSTRACT: We study the statistical-mechanical properties of intertwined double-helical DNAs (DNA braids). In magnetic tweezers experiments, we find that torsionally stressed stretched braids supercoil via an abrupt buckling transition, which is associated with the nucleation of a braid end loop, and that the buckled braid is characterized by a proliferation of multiple domains. Differences between the mechanics of DNA braids and supercoiled single DNAs can be understood as an effect of the increased bulkiness in the structure of the former. The experimental results are in accord with the predictions of a statistical-mechanical model.

SUBMITTER: Brahmachari S

PROVIDER: S-EPMC5726782 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices.

Brahmachari Sumitabha S Gunn Kathryn H KH Giuntoli Rebecca D RD Mondragón Alfonso A Marko John F JF

Physical review letters 20171031 18

We study the statistical-mechanical properties of intertwined double-helical DNAs (DNA braids). In magnetic tweezers experiments, we find that torsionally stressed stretched braids supercoil via an abrupt buckling transition, which is associated with the nucleation of a braid end loop, and that the buckled braid is characterized by a proliferation of multiple domains. Differences between the mechanics of DNA braids and supercoiled single DNAs can be understood as an effect of the increased bulki ...[more]

PMID: 29219598

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