Ontology highlight
ABSTRACT:
SUBMITTER: Schavemaker PE
PROVIDER: S-EPMC5726854 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Schavemaker Paul E PE Śmigiel Wojciech M WM Poolman Bert B
eLife 20171120
Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength. We used surface-modified fluorescent proteins (FPs) and determined their translational diffusion coefficients (<i>D</i>) in the cytoplasm of <i>Escherichia coli</i>, <i>Lactococcus lactis</i> and <i>Haloferax volcanii</i>. We find that in <i>E. coli D</i> depends on the net charge and its distribu ...[more]