Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria.
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ABSTRACT:
SUBMITTER: Bernheim A
PROVIDER: S-EPMC5727150 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
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Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria.
Bernheim Aude A Calvo-Villamañán Alicia A Basier Clovis C Cui Lun L Rocha Eduardo P C EPC Touchon Marie M Bikard David D
Nature communications 20171212 1
Type II CRISPR-Cas systems introduce double-strand breaks into DNA of invading genetic material and use DNA fragments to acquire novel spacers during adaptation. These breaks can be the substrate of several DNA repair pathways, paving the way for interactions. We report that non-homologous end-joining (NHEJ) and type II-A CRISPR-Cas systems only co-occur once among 5563 fully sequenced prokaryotic genomes. We investigated experimentally the possible molecular interactions using the NHEJ pathway ...[more]
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