Ontology highlight
ABSTRACT:
SUBMITTER: Winters IP
PROVIDER: S-EPMC5727199 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Winters Ian P IP Chiou Shin-Heng SH Paulk Nicole K NK McFarland Christopher D CD Lalgudi Pranav V PV Ma Rosanna K RK Lisowski Leszek L Connolly Andrew J AJ Petrov Dmitri A DA Kay Mark A MA Winslow Monte M MM
Nature communications 20171212 1
Large-scale genomic analyses of human cancers have cataloged somatic point mutations thought to initiate tumor development and sustain cancer growth. However, determining the functional significance of specific alterations remains a major bottleneck in our understanding of the genetic determinants of cancer. Here, we present a platform that integrates multiplexed AAV/Cas9-mediated homology-directed repair (HDR) with DNA barcoding and high-throughput sequencing to simultaneously investigate multi ...[more]