Structural insight into catalytic mechanism of PET hydrolase.
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ABSTRACT:
SUBMITTER: Han X
PROVIDER: S-EPMC5727383 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
Structural insight into catalytic mechanism of PET hydrolase.
Han Xu X Liu Weidong W Huang Jian-Wen JW Ma Jiantao J Zheng Yingying Y Ko Tzu-Ping TP Xu Limin L Cheng Ya-Shan YS Chen Chun-Chi CC Guo Rey-Ting RT
Nature communications 20171213 1
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated. ...[more]
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