Ontology highlight
ABSTRACT:
SUBMITTER: Swarbrick CMD
PROVIDER: S-EPMC5728396 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Swarbrick Crystall M D CMD Basavannacharya Chandrakala C Chan Kitti W K KWK Chan Shu-Ann SA Singh Daljit D Wei Na N Phoo Wint Wint WW Luo Dahai D Lescar Julien J Vasudevan Subhash G SG
Nucleic acids research 20171201 22
The protein-RNA interactions within the flavivirus replication complex (RC) are not fully understood. Our structure of dengue virus NS3 adenosine triphosphatase (ATPase)/helicase bound to the conserved 5' genomic RNA 5'-AGUUGUUAGUCU-3' reveals that D290 and R538 make specific interactions with G2 and G5 bases respectively. We show that single-stranded 12-mer RNA stimulates ATPase activity of NS3, however the presence of G2 and G5 leads to significantly higher activation. D290 is adjacent to the ...[more]