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Preclinical modeling of myelodysplastic syndromes.

ABSTRACT: Myelodysplastic syndromes (MDS) represent a heterogeneous group of hematological clonal disorders. Here, we have tested the bone marrow (BM) cells from 38 MDS patients covering all risk groups in two immunodeficient mouse models: NSG and NSG-S. Our data show comparable level of engraftment in both models. The level of engraftment was patient specific with no correlation to any specific MDS risk group. Furthermore, the co-injection of mesenchymal stromal cells (MSCs) did not improve the level of engraftment. Finally, we have developed an in vitro two-dimensional co-culture system as an alternative tool to in vivo. Using our in vitro system, we have been able to co-culture CD34⁺ cells from MDS patient BM on auto- and/or allogeneic MSCs over 4 weeks with a fold expansion of up to 600 times. More importantly, these expanded cells conserved their MDS clonal architecture as well as genomic integrity.

SUBMITTER: Rouault-Pierre K

PROVIDER: S-EPMC5729336 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Preclinical modeling of myelodysplastic syndromes.

Rouault-Pierre K K Mian S A SA Mian S A SA Goulard M M Abarrategi A A Di Tulio A A Smith A E AE Mohamedali A A Best S S Nloga A-M AM Kulasekararaj A G AG Ades L L Chomienne C C Fenaux P P Dosquet C C Mufti G J GJ Bonnet D D

Leukemia 20170602 12

Myelodysplastic syndromes (MDS) represent a heterogeneous group of hematological clonal disorders. Here, we have tested the bone marrow (BM) cells from 38 MDS patients covering all risk groups in two immunodeficient mouse models: NSG and NSG-S. Our data show comparable level of engraftment in both models. The level of engraftment was patient specific with no correlation to any specific MDS risk group. Furthermore, the co-injection of mesenchymal stromal cells (MSCs) did not improve the level of ...[more]

PMID: 28663577

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