DNA damage causes rapid accumulation of phosphoinositides for ATR signaling.
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ABSTRACT:
SUBMITTER: Wang YH
PROVIDER: S-EPMC5730617 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
DNA damage causes rapid accumulation of phosphoinositides for ATR signaling.
Wang Yu-Hsiu YH Hariharan Anushya A Bastianello Giulia G Toyama Yusuke Y Shivashankar G V GV Foiani Marco M Sheetz Michael P MP
Nature communications 20171214 1
Phosphoinositide lipids (PPIs) are enriched in the nucleus and are accumulated at DNA damage sites. Here, we investigate roles of nuclear PPIs in DNA damage response by sequestering specific PPIs with the expression of nuclear-targeted PH domains, which inhibits recruitment of Ataxia telangiectasia and Rad3-related protein (ATR) and reduces activation of Chk1. PPI-binding domains rapidly (< 1 s) accumulate at damage sites with local enrichment of PPIs. Accumulation of PIP<sub>3</sub> in complex ...[more]
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